Overexpression Vector Construction and Bioinformatics Analysis of Dt4CL from Dracocephalum tanguticum Maxim
Why this work is in the frame
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Bibliographic record
Abstract
4-coumarate (4-coumarate: coenzyme A ligase, 4CL) is one of the key enzymes in the biosynthesis of flavonoids and lignin. It is located at the key point of the transition from the main pathway to the branch pathway of phenylalanine metabolism, and controls the entry into different secondary metabolic pathways to produce different secondary metabolites. In this experiment, a gene named Dt4CL1 was cloned from Dracocephalum tanguticum Maxim by PCR. The overexpression vector Dt4CL1-PHB was constructed by double enzyme digestion and plasmid recombination. The protein encoded by the gene was analyzed by bioinformatics, including physical and chemical properties, hydrophobicity, signal peptide, secondary structure, tertiary structure and phylogenetic tree. The results showed that ORF was 1 719 bp in length and encoded 572 amino acids. The predicted molecular weight was 61.61 kD and the theoretical PI was 5.39. Phylogenetic analysis showed that Dt4CL1 protein had the highest homology with the 4CL protein of Salvia miltiorrhiza and Prunella vulgaris. It provides a scientific basis for the study of the function of Dt4CL1 .
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it