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Record W3210699700 · doi:10.1016/j.ymthe.2021.10.008

Single-dose replicating poxvirus vector-based RBD vaccine drives robust humoral and T cell immune response against SARS-CoV-2 infection

2021· article· en· W3210699700 on OpenAlexafffund
Stephen Boulton, Joanna Poutou, Nikolas T. Martin, Taha Azad, Ragunath Singaravelu, Mathieu J. F. Crupi, Taylor R. Jamieson, Xiaohong He, Ricardo Marius, Julia Petryk, Christiano Tanese de Souza, Bradley Austin, Zaid Taha, Jack T. Whelan, S. Khan, Adrian Pelin, Reza Rezaei, Abera Surendran, Sarah Tucker, Emily E.F. Fekete, Jaahnavi Dave, Jean‐Simon Diallo, Rebecca C. Auer, Jonathan B. Angel, D. William Cameron, Jean‐François Cailhier, Réjean Lapointe, Kyle Potts, Douglas J. Mahoney, John C. Bell, Carolina S. Ilkow

Bibliographic record

VenueMolecular Therapy · 2021
Typearticle
Languageen
FieldMedicine
TopicSARS-CoV-2 and COVID-19 Research
Canadian institutionsAlberta Children's HospitalUniversity of CalgaryOttawa HospitalUniversity of Ottawa
FundersMitacsCanadian Institutes of Health ResearchNatural Sciences and Engineering Research Council of CanadaOttawa Hospital FoundationDepartment of Medicine, University of TorontoTerry Fox Research InstituteCanadian Cancer Society
KeywordsImmunogenicityVacciniaVirologyImmune systemCoronavirusVaccinationModified vaccinia AnkaraImmunityAntigenVirusImmunologyBiologyVaccine efficacyViral vectorMedicineDiseaseInfectious disease (medical specialty)Coronavirus disease 2019 (COVID-19)Recombinant DNA

Abstract

fetched live from OpenAlex

The coronavirus disease 2019 (COVID-19) pandemic requires the continued development of safe, long-lasting, and efficacious vaccines for preventive responses to major outbreaks around the world, and especially in isolated and developing countries. To combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), we characterize a temperature-stable vaccine candidate (TOH-Vac1) that uses a replication-competent, attenuated vaccinia virus as a vector to express a membrane-tethered spike receptor binding domain (RBD) antigen. We evaluate the effects of dose escalation and administration routes on vaccine safety, efficacy, and immunogenicity in animal models. Our vaccine induces high levels of SARS-CoV-2 neutralizing antibodies and favorable T cell responses, while maintaining an optimal safety profile in mice and cynomolgus macaques. We demonstrate robust immune responses and protective immunity against SARS-CoV-2 variants after only a single dose. Together, these findings support further development of our novel and versatile vaccine platform as an alternative or complementary approach to current vaccines.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.326
Teacher spread0.280 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations23
Published2021
Admission routes2
Has abstractno

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