Compact Cell Sonoporation Device for Performing Sonoporation Experiments on Adherent Cells
Bibliographic record
Abstract
This paper reports a compact sonoporation device for 24 and 96 well cell culture plates. The proposed device is designed to be used in in vitro studies on adherent cells. K-Wave toolbox was used to model the pressure field and determine a uniform region where cells could be then exposed to ultrasound. The uniformity was confirmed using measurements by a needle hydrophone. The sonoporation device, which included six 30 mm planar transducers, was designed and built. The cell culture plate was placed in the sonoporation device at a height where cells were exposed to uniform acoustic pressure. Three embodiments of the sonoporation device working at 330 kHz, 500 kHz and 1 MHz were built. The results show that more than 80% of the well-area had a measured acoustic pressure at a set value ±25%. The sonoporation device was validated on a dermal mouse fibroblast model. The exposures were performed at 50% duty cycle, 7Hz repetition frequency and pressure values from 0 kPa to 50 kPa for a total exposure time of 1 and 3 minutes. Live/dead viability test showed that the tested conditions did not significantly affect mortality rate, except for 1 MHz, where an increase in death rate was observed.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".