Measuring the effect of environmental stress on cell survival during replication stress
Bibliographic record
Abstract
DNA replication checkpoint ensures cell fitness under replication stress by restraining fork progression and arresting cell cycle. Without checkpoint proteins, cells die in a replication inhibitor hydroxyurea (HU). However, cellular environment may affect their survival in HU. Therefore, the main goal of this study was to examine the effect of environmental stress and to study how it promotes survival in replication checkpoint mutants of fission yeast (rad3∆, mrc1∆, cds1∆). Our viability assays showed a significant increase in these mutants survival in heat-shock + HU compared to HU alone. Cell-cycle staging suggests that cells are altered after heat shock, affecting their response to HU. We measured the consequences to this enhanced survival and found that surviving population exhibits altered DNA mis-segregation and mutation rate. Collectively, our work points to a general cellular response to various environmental stressors that affects survival under replication stress, and may be applicable to human disease.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".