Cloning and Sequence Analysis of <i>GBM5</i> Gene from <i>Ginkgo biloba</i>
Bibliographic record
Abstract
AGAMOUS (AG), a member of MADS-box gene family, is a key gene related to regulation of flower development in plants. The AG homologous gene GBM5 (Accession No.: AY114304.1) was obtained from the transcriptome of Ginkgo biloba . Primers were designed according to the obtained GBM5 gene sequence, and the full-length cDNA sequence of GBM5 gene with 732 bp in size and 243 amino acids in coding product can be synthetized by designing primers. The comparative analysis of evolutionary tree and amino acid sequence showed that ginkgo GBM5 was closely related to cycads AG and black spruce AG . qPCR analysis showed that the expression of GBM5 in different tissues of Ginkgo biloba was different at different periods, the expression levels of different tissues are as follows: Female flower>Male flower>Primordia>Foliage; Its expression is high in the undifferentiated stage of flower bud, the expression patterns were: April leaf>May leaf>September leaf, April primordia>May primordia>September primordia.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".