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Record W3217089033

Investigating Inhibitors of a Phosphatase Enzyme

2021· article· en· W3217089033 on OpenAlexaff
Brooke Corbiere

Bibliographic record

VenueStudent Research Proceedings · 2021
Typearticle
Languageen
FieldMedicine
TopicAlkaline Phosphatase Research Studies
Canadian institutionsMacEwan University
Fundersnot available
KeywordsEnzymeDephosphorylationAlkaline phosphataseSubstrate (aquarium)PhosphataseChemistryNon-competitive inhibitionPhosphateBiochemistryCatalysisEnzyme assayEnzyme catalysisStereochemistryBiology
DOInot available

Abstract

fetched live from OpenAlex

Alkaline phosphatases are enzymes responsible for dephosphorylation of phosphate monoesters. They are involved in numerous biological processes but play a particularly important role in bone development and liver function. Abnormal levels of these enzymes can have detrimental effects on the corresponding processes. This study involved enzyme kinetic studies on bovine alkaline phosphatase to test the effects of substrate and enzyme concentrations, pH, and the addition of known and novel inhibitors. Increased substrate and enzyme concentrations increased the rate of catalysis and allowed for the determination of kinetic constants; Km and Vmax. The optimal pH was determining to be approx. 10, and from comparison of rates of catalysis between uninhibited and inhibited assays, the mode of inhibition was determined to be competitive for the known inhibitors: EDTA and phosphate. Several novel thiazole-based inhibitors with different substitution patterns were also synthesized and tested for their efficacy. Department: Biology Faculty Mentor: Dr. Tina Bott

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.135
GPT teacher head0.460
Teacher spread0.325 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2021
Admission routes1
Has abstractyes

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Same venueStudent Research ProceedingsSame topicAlkaline Phosphatase Research StudiesFrench-language works237,207