Genotype-dependent response of St. John's wort (Hypericum perforatum L.) shoot tips to cryogenic treatment: effect of pre-culture conditions on post-thaw recovery.
Bibliographic record
Abstract
Cryopreservation of medicinal plants is an important tool for restoration of their potential to synthesize valuable secondary metabolites. Establishment of an efficient protocol requires detailed understanding of species/genotype-specific responses to changing external variables. The aim of this work was to study the genotype-dependent response of Hypericum perforatum L. shoot tips to different pre-cryogenic conditions. Shoot apices isolated from plants growing on the hormone-free medium and from clusters induced by addition of benzylaminopurine, were subjected to pre-culture using two agents (0.3M sucrose and 0.076µM abscisic acid) prior to cryopreservation. Although the genotypes represented the seed progeny of one mother plant, the results showed their different capability to withstand cryogenic treatment. The differences were apparent especially in case of plants cultivated on the hormone-free medium. The ability to recover depended also on the pre-culture agent, duration of the pre-culture period and under certain conditions also on interaction between benzylaminopurine and abscisic acid in the medium. The results are illustrated by anatomical alterations of the meristematic zones and adjacent leaf primordia. Long term exposure of cultures to benzylaminopurine resulted in the delay of abscisic acid effect during pre-culture step and thus impaired regeneration capability of shoot apices after cryopreservation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".