The Hsp60 Chaperonin of<i>Legionella pneumophila</i>: an Intriguing Player in Infection of Host Cells
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
This chapter reports that through genetic and functional studies in yeast and mammalian cells, the authors have demonstrates that Legionella pneumophila Hsp60 alters signaling cascades in eukaryotic cells, modifies the actin cytoskeleton of mammalian cells, and alters the trafficking of mitochondria in mammalian cells, making Hsp60 a potential effector in L. pneumophila‘s intracellular establishment. The authors determined that dotA and dotB mutant derivatives of Lp02 had virtually no protease-accessible HtpB; i.e., these mutants displayed no surface-exposed HtpB. In addition, immunogold electron microscopy, performed with an HtpB-specific rabbit antiserum and a secondary gold-conjugated antibody against rabbit immunoglobulin, showed that the Lp02 dot mutants, particularly the dotB mutant, had an increased amount of periplasmic gold particles. This interesting result suggested that a nonfunctional Dot/Icm system results in the accumulation of HtpB in the periplasm. Recombinant HtpB caused Chinese hamster ovary (CHO) cells to lose their stress fibers. The abilities of HtpB to specifically alter eukaryotic signaling pathways, cytoskeletal organization, and organellar traffic are indeed functional characteristics that fit well into HtpB's potential role as an L. pneumophila virulence effector.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it