Label‐Free Cell Migration Assay Using Magnetic Exclusion
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Abstract In vitro wound healing assays are widely used to investigate cell migration during various physiologic and pathologic processes. However, traditional scratch‐based assays produce cell‐free areas that are not reproducible, whereas the alternate insert‐based exclusion method is expensive and time‐consuming. Here, a rapid, label‐free, insert‐free magnetic exclusion technique, where magnetic fields are used to create cell‐free areas is described. Suspensions of diamagnetic cells in a paramagnetic culture medium are seeded into microwells placed on an array of the coaxially arranged ring and cylinder magnets. In the presence of an inhomogeneous field, the magnetic susceptibility difference drives the cells toward an annular region of the lowest field strength. Annular cell aggregates of reproducible dimensions are produced on tissue culture‐treated and collagen I‐fibronectin coated surfaces within 3 h. The effects of the paramagnetic agent on cell viability, metabolism, and gene expression are investigated. A mathematical model reveals the dynamics of the cell‐free area closure. The magnetic exclusion technique is inexpensive, easy to use, and amenable to automation. Potential applications include cancer research, high throughput drug discovery, and screening.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it