Simple, Economical Methods for the Culture of Green Algae for Energy Harvesting from Photosynthesis in a Microfluidic Environment
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Bibliographic record
Abstract
Abstract Ongoing technological advancements continually increase the demand for energy. Among various types of energy harvesting systems, biologically based systems have been an area of increasing interest for the past couple of decades. Such systems provide clean, safe power solutions, mainly for low‐ and ultra‐low‐power applications. The microphotosynthetic power cell (μPSC) is one such system that make use of photosynthetic living cells or organisms to generate power. For strong performance, μPSC technology, because of its interdisciplinary nature, requires optimal engineering of both electrochemical cell design and the culture conditions of the photosynthetic microorganisms. We present here a simple, economical culture method for the photosynthetic microorganism Chlamydomonas reinhardtii suitable for the application of this biologically based power system in any geographical location. This article provides a series of protocols for preparing materials and culture medium designed to facilitate the culture of a suitable C. reinhardtii strain even in a non‐biological laboratory. Possible challenges and methods to overcome them are also discussed. Cultured C. reinhardtii perform sufficiently well that they have already been successfully utilized to generate power from a μPSC, generating a peak power of 200 μW from just 2 ml of exponential‐phase algal culture in a μPSC with an active electrode surface area of 4.84 cm 2 . The μPSC thus has potentially broad applications in low‐ and ultra‐low‐power devices and sensors. © 2021 Wiley Periodicals LLC. This article was corrected on 26 July 2022. See the end of the full text for details. Basic Protocol 1 : Algal growth conditions and algal growth chamber fabrication Basic Protocol 2 : Preparation of Tris‐acetate‐phosphate (TAP) nutrient medium Basic Protocol 3 : Preparation of suspension algal culture from algal strain Basic Protocol 4 : Preparation of stock culture plates (algal strain) from suspension algal culture
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it