ZO-1 regulates stress granule formation in endothelial cells by interacting with YB-1
Bibliographic record
Abstract
Abstract Zonal occludens-1 (ZO-1) is involved in the regulation of cell-cell junctions between endothelial cells (ECs). Here, we define the ZO-1 protein interactome and reveal novel ZO-1 interactions with RNA binding proteins (RBP) that are part of stress granules (SGs). Exposure of ECs to stresses decreased ZO-1 protein levels which was accompanied by increased SG formation and protection of ECs from cellular insults. We uncovered a previously undocumented association between ZO-1 and Y-box Binding Protein 1 (YB-1). Arsenite treatment of ECs decreased the interaction between ZO-1 and YB-1. In turn, downregulation of ZO-1 increased the association of YB-1 and G3BP1, thus driving SG assembly. Importantly, YB-1 is essential for the protective effects induced by downregulation of ZO-1 and SG formation. Finally, in the developing retinal vascular plexus of newborn mice, ECs at the extremities of growing vessels express less ZO-1 and display more YB-1 positive SGs than ECs located in the median portion of the retinal vasculature. Together, our findings demonstrate an unsuspected role for ZO-1 in SG formation, through its interaction with YB-1, and in the response of ECs to stresses during angiogenesis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".