ZO-1 regulates stress granule formation in endothelial cells by interacting with YB-1
Bibliographic record
Abstract
<title>Abstract</title> Zonal occludens-1 (ZO-1) is involved in the regulation of cell-cell junctions between endothelial cells (ECs). Here, we define the ZO-1 protein interactome and reveal novel ZO-1 interactions with RNA binding proteins (RBP) that are part of stress granules (SGs). Exposure of ECs to stresses decreased ZO-1 protein levels which was accompanied by increased SG formation and protection of ECs from cellular insults. We uncovered a previously undocumented association between ZO-1 and Y-box Binding Protein 1 (YB-1). Arsenite treatment of ECs decreased the interaction between ZO-1 and YB-1. In turn, downregulation of ZO-1 increased the association of YB-1 and G3BP1, thus driving SG assembly. Importantly, YB-1 is essential for the protective effects induced by downregulation of ZO-1 and SG formation. Finally, in the developing retinal vascular plexus of newborn mice, ECs at the extremities of growing vessels express less ZO-1 and display more YB-1 positive SGs than ECs located in the median portion of the retinal vasculature. Together, our findings demonstrate an unsuspected role for ZO-1 in SG formation, through its interaction with YB-1, and in the response of ECs to stresses during angiogenesis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.002 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".