Bibliographic record
Abstract
水稻矮缩病毒(RDV)的基因组包含12条双链RNA, 其中基因组片段S6编码的非结构蛋白Pns6为该病毒的运动蛋白. 在本研究中, 在大肠杆菌中表达了N端融合His-tag的重组Pns6蛋白. 在低温和低IPTG浓度的诱导后, 通过Ni螯合亲和层析进行纯化获得了HisPns6蛋白. 稳定性分析表明, HisPns6是一个稳定的蛋白, 可耐受37℃下长达24 h的处理. 纯化的蛋白后续用于单克隆抗体的制备并得到18个杂交瘤细胞株系. 使用从感染RDV的水稻叶片中提取的Pns6为样品, 以健康水稻总蛋白为对照, 通过Western blot法对抗体进行特异性分析, 获得了15个阳性抗体. 对其进行抗原决定簇分析表明, 最敏感的抗原决定簇位于Pns6的C端区域(296~509位氨基酸). 该结果与生物信息学预测分析相吻合.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.007 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".