Bibliographic record
Abstract
水稻矮缩病毒(RDV)的基因组包含12条双链RNA, 其中基因组片段S6编码的非结构蛋白Pns6为该病毒的运动蛋白. 在本研究中, 在大肠杆菌中表达了N端融合His-tag的重组Pns6蛋白. 在低温和低IPTG浓度的诱导后, 通过Ni螯合亲和层析进行纯化获得了HisPns6蛋白. 稳定性分析表明, HisPns6是一个稳定的蛋白, 可耐受37℃下长达24 h的处理. 纯化的蛋白后续用于单克隆抗体的制备并得到18个杂交瘤细胞株系. 使用从感染RDV的水稻叶片中提取的Pns6为样品, 以健康水稻总蛋白为对照, 通过Western blot法对抗体进行特异性分析, 获得了15个阳性抗体. 对其进行抗原决定簇分析表明, 最敏感的抗原决定簇位于Pns6的C端区域(296~509位氨基酸). 该结果与生物信息学预测分析相吻合.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.005 |
| Science and technology studies | 0.001 | 0.004 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.003 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".