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Record W4211120128 · doi:10.1128/9781555816841.ch9

Osmotic Stress

2014· book-chapter· en· W4211120128 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueASM Press eBooks · 2014
Typebook-chapter
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBacterial Genetics and Biotechnology
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsOsmoregulationOsmotic shockOsmotic pressureBiologyOsmotic concentrationMechanosensitive channelsBiochemistryTranscription (linguistics)Cell biologyGeneSalinityIon channelEcology

Abstract

fetched live from OpenAlex

Osmotic stress tolerance mechanisms determine whether bacteria survive or grow because osmotic stress profoundly affects the structure, physics, and chemistry of bacterial cells. In vitro studies have shown that K+ glutamate differentially modulates transcription mediated by the σ70 and σS RNA polymerases of Escherichia coli, the latter being central to many stress response. Progress toward understanding the structural changes associated with the opening of representative channels is discussed in this chapter. The study of osmoregulatory proteins is motivated partly by a desire to understand how cells sense osmotic pressure (osmosensing). During the last decade, representative osmoregulatory transporters and mechanosensitive (MS) channels have been shown to both sense osmotic pressure changes (osmosensing) and respond by modulating transmembrane solute distribution (osmoregulation) after purification and reconstitution in proteoliposomes. The osmoregulation of protein activity is discussed by focusing on representative proteins that have been studied. In many bacteria, the proportion of anionic phospholipids increases and the fatty acid composition changes with cultivation at high salinity. For E. coli, growth at high osmolality increases the proportion of CL at the expense of PE without changing the proportion of PG or the fatty acid composition. Interest in the osmoregulation of transcription was stimulated by a desire to understand how osmolality can direct gene expression. Studies focused on promoter identification and the identification of transcriptional regulatory proteins were complicated by multiple factors, including transcription that depends on multiple promoters and σ factors.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.794
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.212
Teacher spread0.198 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it