Osmoregulation in<i>Bacillus subtilis</i>under potassium limitation: a new inducible K<sup>+</sup>-stimulated, VO<sub>4</sub><sup>3</sup>-inhibited ATPase
Bibliographic record
Abstract
Bacillus subtilis exhibited an inducible K + -transporting ATPase activity with apparent K m and maximum velocity V max of 12.9 µM and 25.1 µmol·min 1 ·(g cell protein) 1 , respectively, when cultivated on a synthetic medium containing less than 400 µM K + . Due to this enzyme, the growth rate of the bacterium in synthetic medium was not changed down to 115 µM K + , and the bacterium was able to grow down to 20 µM K + . The limiting K + concentration was higher in media with osmolarity increased by NaCl or sucrose. The ATPase was inhibited by micromolar concentrations of vanadate (K i = 1.6 µM). The ATPase activity was not stimulated by any other monovalent cation. The subunit of this ATPase, with an M r of 52 000, covalently bound the gamma phosphate group of ATP. This phosphorylated intermediate was unstable in neutral and basic pH as well as in the presence of potassium and was stable in acid pH. The enzyme did not show immunological cross-reactivity with antibody against Kdp ATPase of Escherichia coli.Key words: Kdp-like, potassium transport, Bacillus subtilis, transport ATPase, P-type ATPase.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".