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Record W4214763269 · doi:10.21271/zjpas.34.1.9

Direct identification of fungi associated with indoor and outdoor ornamental plants by utilizing PCR assay

2022· article· en· W4214763269 on OpenAlexaboutno aff
rezhna majed, Qasim Abdullah Marzani

Bibliographic record

VenueZANCO Journal of Pure and Applied Sciences · 2022
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant Pathogens and Fungal Diseases
Canadian institutionsnot available
Fundersnot available
KeywordsOrnamental plantIdentification (biology)BiologyEnvironmental scienceBotany

Abstract

fetched live from OpenAlex

Infection by phytopathogenic fungal species can result in significant losses in ornamental plants. For accurate diagnosis of plant diseases, DNA-based techniques have become dependent method. This study aimed to elaborate Polymerase chain reaction (PCR) for rapid detection of fungal pathogens causing diseases on ornamental plants. In this study, specific primers were used to screen suspected plant diseases. The results revealed that Alternaria, Cylindrocladium, Myrothecium, Sclerotium, Cercospora, and Bipolaris, were the most abundant fungi that infect indoor or outdoor ornamental plants. From which, Alternaria alternata, Cylindrocladium sp., and Myrothecium sp. were found to be the most frequent fungi on foliage of these plants. REFERENCES ABBAS, M. F., RAFIQ, M., AL-SADI, A. M., ALFARRAJ, S., ALHARBI, S. A., ARIF, M. & ANSARI, M. J. 2021. Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale. Plos one, 16, e0251471. AGRIOS, A. G. & PICHAT, P. 2005. State of the art and perspectives on materials and applications of photocatalysis over TiO 2. Journal of Applied Electrochemistry, 35, 655-663. AGRIOS, G. 2004. Fitopatología. 2da Edicion. Limusa. México, DF 635p. AUTHORITY, E. F. S. 2010. 2008 Annual Report on Pesticide Residues according to Article 32 of Regulation (EC) No 396/2005. EFSA Journal, 8, 1646. BAKHSHI, M., ARZANLOU, M. & BABAI-AHARI, A. 2012. Morphological and molecular characterization of Cercospora zebrina from black bindweed in Iran. Plant Pathology and Quarantine, 2, 125-130. BILLAH, K. M. 2017. Pathogenicity of Sclerotium rolfsii on different host, and its over wintering survival; A mini review. International Journal of Advances in Agriculture Sciences, 2 (1). BOONHAM, N., SMITH, P., WALSH, K., TAME, J., MORRIS, J., SPENCE, N., BENNISON, J. & BARKER, I. 2002. The detection of Tomato spotted wilt virus (TSWV) in individual thrips using real time fluorescent RT-PCR (TaqMan). Journal of virological methods, 101, 37-48. BOSTAN, H., NIE, X. & SINGH, R. P. 2004. An RT-PCR primer pair for the detection of Pospiviroid and its application in surveying ornamental plants for viroids. Journal of Virological Methods, 116, 189-193. CHASE, A. R. 1997. Foliage plant diseases: diagnosis and control, American Phytopathological Society (APS Press). FANG, Y. & RAMASAMY, R. P. 2015. Current and prospective methods for plant disease detection. Biosensors, 5, 537-561. FEAU, N., BEAUSEIGLE, S., BERGERON, M.-J., BILODEAU, G. J., BIROL, I., CERVANTES-ARANGO, S., DHILLON, B., DALE, A. L., HERATH, P. & JONES, S. J. 2018. Genome-Enhanced Detection and Identification (GEDI) of plant pathogens. PeerJ, 6, e4392. GUNDERSEN, D. & LEE, I.-M. 1996. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia mediterranea, 144-151. HARIHARAN, G. & PRASANNATH, K. 2021. Recent advances in molecular diagnostics of fungal plant pathogens: a mini review. Frontiers in Cellular and Infection Microbiology, 10, 829. HIROOKA, Y., TAKEUCHI, J., HORIE, H. & NATSUAKI, K. T. 2008. Cylindrocladium brown leaf spot on Howea belmoreana caused by Calonectria ilicicola (anamorph: Cylindrocladium parasiticum) in Japan. Journal of General Plant Pathology, 74, 66-70. JAFARI, M., MINAEI, S. & SAFAIE, N. 2017. Detection of pre-symptomatic rose powdery-mildew and gray-mold diseases based on thermal vision. Infrared Physics & Technology, 85, 170-183. JOCK, S., RODONI, B., GILLINGS, M., KIM, W.-S., COPES, C., MERRIMAN, P. & GEIDER, K. 2000. Screening of ornamental plants from the Botanic Gardens of Melbourne and Adelaide for the occurrence of Erwinia amylovora. Australasian Plant Pathology, 29, 120-128. JYAN, M.-H., HUANG, L.-C., ANN, P.-J. & LIOU, R.-F. 2002. Rapid detection of Phytophthora infestans by PCR. Plant Pathology Bulletin, 11, 45-56. KERNAGHAN, G., REELEDER, R. & HOKE, S. 2008. Quantification of Pythium populations in ginseng soils by culture dependent and real-time PCR methods. applied soil ecology, 40, 447-455. LARRAÑAGA, P., DÍAZ-DELLAVALLE, P., CABRERA, A., ALEM, D., LEONI, C., SOUZA, A. L. A. & DE SIMONE, S. G. 2012. Activity of naturally derived antimicrobial peptides against filamentous fungi relevant for agriculture. LUCHI, N., IOOS, R. & SANTINI, A. 2020. Fast and reliable molecular methods to detect fungal pathogens in woody plants. Applied microbiology and biotechnology, 104, 2453-2468. MA, Z. & MICHAILIDES, T. J. 2007. Approaches for eliminating PCR inhibitors and designing PCR primers for the detection of phytopathogenic fungi. Crop Protection, 26, 145-161. MATIĆ, S., GILARDI, G., GULLINO, M. L. & GARIBALDI, A. 2019. Emergence of leaf spot disease on leafy vegetable and ornamental crops caused by Paramyrothecium and Albifimbria species. Phytopathology, 109, 1053-1061. MATIĆ, S., TABONE, G., GARIBALDI, A. & GULLINO, M. L. 2020. Alternaria leaf spot caused by Alternaria species: an emerging problem on ornamental plants in Italy. Plant Disease, 104, 2275-2287. MCCUISTON, J. L., HUDSON, L. C., SUBBOTIN, S. A., DAVIS, E. L. & WARFIELD, C. Y. 2007. Conventional and PCR detection of Aphelenchoides fragariae in diverse ornamental host plant species. Journal of Nematology, 39, 343. MMBAGA, M., KIM, M.-S., MACKASMIEL, L. & KLOPFENSTEIN, N. 2015a. Differentiation of Corynespora cassiicola and Cercospora sp. in leaf-spot diseases of Hydrangea macrophylla using a PCR-mediated method. Canadian Journal of Plant Science, 95, 711-717. MMBAGA, M., KIM, M., MACKASMIEL, L. & KLOPFEINSTEIN, N. 2015b. Differentiation of Corynespora cassiicola and Cercospora sp. In leaf-spot diseases of Hydrangea…. Charges: A charge of $90.00 per page, plus the cost of tables, figures, and abstract translation. MMBAGA, M., LI, Y. & KIM, M.-S. 2010. First report of Myrothecium roridum causing leaf spot on garden hydrangea in the United States. Plant disease, 94, 1266-1266. PALLÁS, V., SÁNCHEZ-NAVARRO, J. A. & JAMES, D. 2018. Recent advances on the multiplex molecular detection of plant viruses and viroids. Frontiers in microbiology, 9, 2087. PUERTOLAS, A., BONANTS, P. J., BOA, E. & WOODWARD, S. 2021. Application of real-time PCR for the detection and quantification of oomycetes in ornamental nursery stock. Journal of Fungi, 7, 87. PUNJA, Z. K., DE BOER, S. & SANFAÇON, H. 2007. Biotechnology and plant disease management, Cabi. RIGOTTI, S., GINDRO, K. & VIRET, O. 2006. Two New Primers Highly Specific for the Detection of" Botrytis cinerea" Pers. Fr. Two New Primers Highly Specific for the Detection of" Botrytis cinerea" Pers. Fr., 1000-1008. RILEY, M. B., WILLIAMSON, M. R. & MALOY, O. 2002. Plant disease diagnosis. The Plant Health Instructor, 10. SAVÉ, R. What is stress and how to deal with it in ornamental plants? VI International Symposium on New Floricultural Crops 813, 2007. 241-254. SCHIPPERS, B. & GAMS, W. 1979. Soil-borne plant pathogens, Academic Press. SHI, A., MMBAGA, M. T., MREMA, F. & NNODU, E. 2005. Molecular Analysis of Alternaria alternata in Lilac. SHUPING, D. T. T. & ELOFF, J. T. 2017. The use of plants to protect plants and food against fungal pathogens: A review. African Journal of Traditional, Complementary and Alternative Medicines, 14, 120-127. SKOLIK, P. 2020. Sensor based pre-symptomatic detection of pests and pathogens for precision scheduling of crop protection products. Lancaster University. STUTZ, M. C., CARRER FILHO, R., ROCHA, G. A., DE CAMPOS DIANESE, É. & DA CUNHA, M. G. 2019. Soft rot on the stems of Zamioculcas zamiifolia caused by Sclerotium rolfsii. Ornamental Horticulture, 25, 402-406. TRKULJA, N., MILOSAVLJEVIĆ, A., ŽIVKOVIĆ, S., POPOVIĆ, T., MITROVIĆ, M., JOVIĆ, J. & TOŠEVSKI, I. 2015. First report of Cercospora violae infecting the garden violet Viola odorata in Serbia. Plant Disease, 99, 1035. VAN DEN BOSCH, F., AKUDIBILAH, G., SEAL, S. & JEGER, M. 2006. Host resistance and the evolutionary response of plant viruses. Journal of Applied Ecology, 43, 506-516.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.153
Threshold uncertainty score0.194

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.227
Teacher spread0.216 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2022
Admission routes1
Has abstractyes

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Same venueZANCO Journal of Pure and Applied SciencesSame topicPlant Pathogens and Fungal DiseasesFrench-language works237,207