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Record W4220802585 · doi:10.21203/rs.3.rs-1503219/v1

First Report of Cryptosporidium spp. in Cattle from Jamaica

2022· preprint· en· W4220802585 on OpenAlexaff
Amanda Morris, Simmoy Noble, John Lindo, Corinne Ong

Bibliographic record

VenueResearch Square · 2022
Typepreprint
Languageen
FieldImmunology and Microbiology
TopicParasitic Infections and Diagnostics
Canadian institutionsToronto Metropolitan University
Fundersnot available
KeywordsCryptosporidiumBiologyFecesZoonosisCryptosporidium parvumPolymerase chain reactionVeterinary medicineLivestockNested polymerase chain reactionCattle DiseasesParasite hostingTransmission (telecommunications)ParasitologyMicrobiologyVirologyZoologyGeneEcologyGeneticsMedicine

Abstract

fetched live from OpenAlex

Abstract Background: Cryptosporidium is a protozoan parasite associated with gastrointestinal illness in humans and a wide range of vertebrate animals. Cattle, particularly calves, are widely recognized as major reservoir hosts of zoonotic Cryptosporidium spp. Despite this, and cattle being one of the most significant economic mainstays in St. Elizabeth, Jamaica, there is no publish record on the presence of Cryptosporidium in Jamaican cattle nor their potential role as zoonotic reservoirs. Methods: In the present study, 119 fecal specimens were collected from dairy and beef cattle from 10 farms in St. Elizabeth parish. Polymerase chain reaction (PCR), amplifying the 18S rRNA gene, confirmed Cryptosporidium presence in 10 (8 %) of samples. Results: Sequence analysis of PCR products identified three C. parvum isolates (previously reported in calves) and one C. hominis isolate among calves experiencing diarrhea at the time of sample collection. Further nested PCR and sequence analysis of the 60 kDa glycoprotein (gp60) gene, identified the C. hominis IbA9G2 subtype, previously reported in a calf. Conclusions: This reports the first molecular characterization of Cryptosporidium spp. from cattle in Jamaica, and their potential role as zoonotic transmitters of the protozoan parasite. Further molecular investigation is warranted to better understand direct and indirect transmission dynamics of cryptosporidiosis between livestock and humans on the island.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Case report · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.067
Threshold uncertainty score0.134

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0020.001
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.374
Teacher spread0.329 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designCase report
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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