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Record W4220946319 · doi:10.3390/toxins14030224

Screening of Pig-Derived Zearalenone-Degrading Bacteria through the Zearalenone Challenge Model, and Their Degradation Characteristics

2022· article· en· W4220946319 on OpenAlexaff
Xue Yang, Feng Li, Hangyi Ning, Wei Zhang, Yan D. Niu, Zhuo Shi, Sa Chai, Anshan Shan

Bibliographic record

VenueToxins · 2022
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicMycotoxins in Agriculture and Food
Canadian institutionsUniversity of Calgary
FundersNational Key Research and Development Program of ChinaNatural Science Foundation of Heilongjiang Province
KeywordsZearalenoneToxicityMicrobiologyBacteriaProteus mirabilisBacillus subtilisDegradation (telecommunications)Food scienceIntracellularStrain (injury)BiologyChemistryBiodegradationMycotoxinBiochemistry

Abstract

fetched live from OpenAlex

Zearalenone (ZEN) is widely found in food and feed. Its cytotoxicity, reproductive toxicity, genetic toxicity, immunotoxicity and hepatorenal toxicity have serious impacts on human and animal health. In order to help animals avoid ZEN poisoning in feed, ZEN-degrading bacterial strains were screened from fecal samples through a zearalenone challenge pig model, and their degradation characteristics were researched. Through the optimization of parameters such as the culture time, pH value, temperature, and strain concentration, the optimal conditions for the ZEN-degrading ability of these strains were preliminarily determined, and the active site of the ZEN degradation was explored. In this study, three strains (SY-3, SY-14, SY-20) with high ZEN degradation capacities were obtained. SY-3 was identified as Proteus mirabilis, and its main degrading component was the supernatant. SY-14 and SY-20 were identified as Bacillus subtilis. Their main degrading components were the intracellular fluid of SY-14, and the intracellular fluid and cell wall of SY-20. The above results showed that the ZEN challenge model was an effective way to screen ZEN-degrading bacteria.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.039
GPT teacher head0.219
Teacher spread0.180 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations22
Published2022
Admission routes1
Has abstractyes

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