A Study of Karabaghian Skullcap (Scutellaria platystegia Juz.): Antioxidant and Antibacterial Activity Assays, Essential Oil Analysis, and Isolation of Its Phenolic Compounds
Bibliographic record
Abstract
Background: Scutellaria platystegia Juz. is an herbaceous perennial, distributed in the northwest of Iran and southern Caucasian countries? Objectives: This study aimed to examine the antioxidant and antibacterial activities of the aerial part of S. platystegia and to determine the phytochemical constituents of its extract and essential oil. Methods: The ferric reducing ability of plasma (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods were used to examine the antioxidant effects of fractions obtained from the hydroalcoholic extract of the aerial part of S. platystegia. The antibacterial activity screening was also performed using the disk diffusion and microdilution methods. The phytochemical constituents of the most active fractions were investigated using column chromatography. Nuclear magnetic resonance (NMR) imaging and UV-Vis spectral analysis were used to determine the chemical structure of the isolated compounds. The GC-MS technique was also employed to identify the essential oil composition of the plant. Results: In the antioxidant activity assay, the n-butanol fraction was the most potent fraction, with a half maximal inhibitory concentration (IC50) of 16.14 ± 0.8 µg/mL on the DPPH assay and 736.4 ± 4.6 mmol FeSO4.7H2O equivalent per gram of sample on the FRAP assay. The n-butanol fraction exhibited very strong antibacterial activities against Staphylococcus aureus, Staphylococcus epidermidis, and Shigella dysenteriae (inhibition zone diameter: 20 - 25 mm, MIC: 125 - 250 µg/mL). The phytochemical analysis of the most active fractions resulted in the isolation of the following components from the chloroform fraction: 5,7,2’-trihydroxy-8,6’-dimethoxyflavone; 5-hydroxy-6,7,8,2’,6’-pentamethoxyflavone; 5,2’,6’-trihydroxy-7,8-dimethoxyflavone; 5,2’,6’-trihydroxy-6,7,8-trimethoxyflavone; and 5,4’-dihydroxy-7-methoxyflavone. Besides, the following components were extracted from the n-butanol fraction: luteolin-7-O-β-D-glucopyranoside; verbascoside; apigenin; kaempferol; caffeic acid; rosmarinic acid; apigenin-7-O-β-D-glucopyranoside; apigenin-7-O-β-D-(-6’’-(E)-caffeoyl)-glucopyranoside; and luteolin. Fourteen compounds were also identified in the plant essential oil; terpinen-4-ol (44.41%), α-terpineol (10.75%), caryophyllene oxide (9.61%), and thymol (8.73%) were the main compounds. Conclusions: This study introduced S. platystegia as a plant rich in bioactive phenolic compounds, with considerable antioxidant and antimicrobial activities. Therefore, it can be suggested as a potential candidate to be evaluated in future biological studies.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.003 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".