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Record W4225139261 · doi:10.1016/j.isci.2022.104335

Highly transmissible cytoplasmic incompatibility by the extracellular insect symbiont Spiroplasma

2022· article· en· W4225139261 on OpenAlexafffund
Marie Pollmann, Logan D. Moore, Elena Krimmer, Paul D’Alvise, Martin Hasselmann, Steve J. Perlman, Matthew J. Ballinger, Johannes L. M. Steidle, Yuval Gottlieb

Bibliographic record

VenueiScience · 2022
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicInsect symbiosis and bacterial influences
Canadian institutionsUniversity of Victoria
FundersNational Institute of General Medical SciencesNatural Sciences and Engineering Research Council of CanadaUniversität HohenheimMinisterium für Wissenschaft, Forschung und Kunst Baden-WürttembergHebrew University of JerusalemNational Institutes of HealthSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
KeywordsSpiroplasmaWolbachiaBiologyGenomeInsectMatingGeneGeneticsHost (biology)Cytoplasmic incompatibilityEvolutionary biologyMollicutesBacteriaBotany

Abstract

fetched live from OpenAlex

Cytoplasmic incompatibility (CI) is a form of reproductive manipulation caused by maternally inherited endosymbionts infecting arthropods, like Wolbachia , whereby matings between infected males and uninfected females produce few or no offspring. We report the discovery of a new CI symbiont, a strain of Spiroplasma causing CI in the parasitoid wasp Lariophagus distinguendus . Its extracellular occurrence enabled us to establish CI in uninfected adult insects by transferring Spiroplasma -infected hemolymph. We sequenced the CI- Spiroplasma genome and did not find any homologues of any of the cif genes discovered to cause CI in Wolbachia , suggesting independent evolution of CI. Instead, the genome contains other potential CI-causing candidate genes, such as homologues of high-mobility group (HMG) box proteins that are crucial in eukaryotic development but rare in bacterial genomes. Spiroplasma 's extracellular nature and broad host range encompassing medically and agriculturally important arthropods make it a promising tool to study CI and its applications.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.000
Threshold uncertainty score0.001

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.211
Teacher spread0.189 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations66
Published2022
Admission routes2
Has abstractyes

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