Complex II‐linked mitochondrial respiration is upregulated during postnatal development in high‐altitude grown mice
Bibliographic record
Abstract
Recently, we have shown that chronic normobaric hypoxia (12% O 2 – 21 days) triggers mitochondrial and cell‐ metabolic plasticity in the retrosplenial cortex of male adult FVB mice but not in SD rats. In these animals we reported a transient upregulation of anaerobic metabolic pathways (glycolysis + lactate metabolism) followed by an attenuation in the mitochondrial respiration. These results pointed to an optimized use of oxygen at cellular level in an area of the brain responsible of processing visual information and spatial memory. Features of great importance in the biological fitness. In the current work we investigate how the residence in high altitude (La Paz – Bolivia, 3,600 m) affects the developmental profile of the mitochondrial respiratory efficiency in the retrosplenial cortex of mice and rats. We hypothesize that the mitochondrial plasticity observed in adult mice will have a process of maturation during postnatal development. This response shall be absent in rats. To do so, we measured the mitochondrial oxygen consumption rate (OCR) in saponin‐permeabilized brain samples from FVB mice and SD rats born and grown in La Paz. The animals were sacrificed at postnatal ages p7, p14, p21, and adulthood (P90), and the OCR linked to the use of NADH (N pathway), FADH 2 (S pathway), or both (NS pathway) was quantified by means of high‐resolution respirometry (OROBOROS). Our results show a pattern of maturation (increase) of the mitochondrial respiration in the retrosplenial cortex along with the postnatal development (21 days+) of mice and rats. However, an upregulation of the participation of the S pathway (complex II‐linked) in the mitochondrial respiration was observed during the first three weeks of age only in mice. This observation supports previous works suggesting a key role of the complex II and succinate (substrate of complex II) in the successful cellular and mitochondrial acclimatization to hypoxia. These findings contribute to further establish FVB mice and SD rats as a model to study divergent acclimatization to hypoxia.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".