Potential of spore protein profiles as identification tools for arbuscular mycorrhizal fungi
Bibliographic record
Abstract
Five hundred spores from monospecific AMF cultures of Glomus clarum, G. mosseae, G. versiforme, G. fasciculatum, G. etunicatum, and a Glomus sp. isolate BVB1 were crushed in a modified sodium dodecyl sulphate (SDS) reducing buffer and 0.5 μg spore protein aliquots were subjected to one dimensional SDS polyacrylamide gel electrophoresis (1D SDS-PAGE). Silver-stained gels revealed a unique and reproducible protein profile for each of the AMF species tested. The average similarity between any two AMF species ranged ca 10–51%, as determined using an unweighted pair-group average approach. Furthermore, each of the AMF species possessed signature protein bands that were reproducible and consistent. SDS-PAGE of AMF spores is a simple and sensitive technique capable of distinguishing between AMF species that could be used for the routine identification of unknown AMF isolates.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".