Differentiation of infection structures of the powdery mildew fungus<i>Uncinula necator</i>and adhesion to the host cuticle
Bibliographic record
Abstract
Development and adhesion of infection structures of the grapevine powdery mildew fungus, Uncinula necator (Schw.) Burr., were investigated during the early stages of leaf colonization. Light microscopy showed that primary appressoria occurred 3.5 h post inoculation (p.i.) and that hyphae on the leaf surface, indicative of successful host colonization, appeared 14 h p.i. Low temperature scanning electron microscopy revealed deposits of extracellular material at the contact zone of fungal structures and plant cuticle, suggesting firm attachment of the pathogen. To investigate whether or not esterase or cutinase activity is involved in establishing the fungus on the host cuticle, histochemical assays and inhibitor studies were performed. Results indicated that esterase activity was associated with conidia and infection structures. A single fungal extracellular protein was identified as a cutinase by its ability to hydrolyze3H-cutin. Probing Southern blots of genomic DNA of U. necator, Magnaporthe grisea, and Fusarium solani f.sp. pisi with the cutinase gene of F. solani f.sp. pisi suggested that the cutinase gene of U. necator shares only limited sequence similarities with the cutinase genes of the other fungi investigated. Adhesion assays showed that the presence of esterase-cutinase inhibitors on the cuticle did not significantly affect adhesion. The role of the enzyme in fungal adhesion is discussed.Key words: grapevine powdery mildew, Vitis vinifera, cutinase, extracellular matrix, cryofixation, low temperature scanning electron microscopy.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".