Hormonal activation of phosphorylase in cockroach fat body trophocytes: A correlation with trans‐membrane calcium flux
Bibliographic record
Abstract
Abstract This study is an investigation of the temporal relationship between transmembrane Ca 2+ fluxes, and glycogen phosphorylase activation in dispersed trophocytes from the fat body of the cockroach, Periplaneta americana . Phosphorylase is maximally activated within 5 min after treating the trophocytes with either of the hypertrehalosemic hormones, Pea‐HTH‐I and Pea‐HTH‐II. Activation caused by Pea‐HTH‐II is sustained for a longer period than that produced by Pea‐HTH‐I. Chelation of extracellular Ca 2+ with EGTA blocks the activation of phosphorylase by HTH. Similarly, chelation of intracellular Ca 2+ with Quin 2 greatly diminishes the phosphorylase activating effect of both HTHs. The data support the view that an increase in the intracellular Ca 2+ concentration is required for the activation of phosphorylase and that extracellular Ca 2+ is an essential, although not necessarily sole, source of Ca 2+ for this purpose. Using 45 Ca 2+ to trace the movement of Ca 2+ following a challenge with either Pea‐HTH‐I or ‐II, it was shown that 45 Ca 2+ influx nearly doubled during the first 30 s. At this time, the trophocytes begin to expel Ca 2+ at a rate higher than that of untreated cells and this state persists for approximately 4 min. The Ca 2+ fluxes are consistent with its postulated role in the activation of phosphorylase. Arch. Insect Biochem. Physiol. 42:233–244, 1999. © 1999 Wiley‐Liss, Inc.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".