Eighth International Conference on the Juvenile Hormones
Bibliographic record
Abstract
The biosynthesis of juvenile hormone (JH) by the corpora allata (CA) can be either stimulated or inhibited by neuropeptides termed allatotropin (AT) or allatostatin (AS), respectively.To date, only one AT has been identified.It was first isolated from heads of pharate adults of Manduca sexta (Manse-AT; GFKNVEMMTARGF-NH2).The gene encoding Manse-AT has been cloned from various lepidopterans, including S. frugiperda.Here we report the identification of a second peptide with allatotropic activity from the brain of S. frugiperda by molecular techniques.A cDNA that encodes 53 amino acids included one copy of the RVRG NPISCF-OH peptide.This peptide strongly stimulated the synthesis and release of JH in vitro by the CA of female adult S. frugiperda and was code-named Spofr-AT 2. The stimulation was dose-dependent with an app.EC 50 of ca. 10 -7 M. CA that were activated with Spofr-AT 2 could be inhibited by the addition of Manse-AS to the incubation medium.Northern blotting and RT-PCR analyses revealed that the preprohormone is expressed in the brain, midgut, and ovary in a developmental-specific manner.Whole-mount in situ hybridisation confirmed the gene expression in various tissues of adult females.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.003 | 0.002 |
| Open science | 0.001 | 0.002 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.081 | 0.054 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".