2,4-dinitrophenol acutely inhibits rabbit atrial Ca<sup>2+</sup>-sensitive Cl<sup>-</sup> current (I<sub>TO2</sub>)
Bibliographic record
Abstract
We investigated the effects of 2,4-dinitrophenol (DNP), the uncoupler of mitochondrial oxidative phosphorylation, on the Ca2+-sensitive Cl- current component of the transient outward current (ITO2). Amphotericin B perforated-patch, whole-cell patch-clamp technique was employed (35°C) using enzymatically isolated single rabbit atrial myocytes. We defined ITO2 as the amplitude of the 2 mM 4-aminopyridine resistant transient outward current sensitive to anthracene-9-carboxylic acid (A9C). Between +5 and +45 mV, 0.2 mM A9C inhibited ITO2 by ~70% (n = 13). Within 30 s after application of 0.2 mM DNP, both normal ITO2 transients (n = 8) and the ITO2 transients that remained after A9C treatment (n = 8) were inhibited completely. In cells expressing ITO2 (70% of total), DNP also suppressed an A9C-insensitive slow outward current by ~40%, but the holding current at -80 mV was unaffected. There was a ~2 min latency between inhibitory effects of DNP and subsequent membrane current increase, presumably caused by activation of the ATP-sensitive K+ channels (n = 16). We conclude that DNP acutely inhibits ITO2 via a mechanism presumably separate from metabolic inhibition.Key words: patch clamp, rabbit heart, simulated ischemia, calcium-sensitive chloride current.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".