Fourth international workshop on the CCN family of genes: Abstracts and posters October 18–21, 2006
Bibliographic record
Abstract
The CCN2 gene is strongly induced in smooth muscle-rich hollow organs which experience mechanical overload arising from either hypertension, altered hemodynamics or obstruction.This suggests that mechanical forces might be the primary inducers of the CCN2 gene in smooth muscle cells.In this study, we found that CCN2 gene expression is rapidly induced in cyclically stretched bladder smooth muscle cells (SMCs) in vitro, and in the detrusor muscle of a mechanically overloaded bladder in a rat model of experimental urethral obstruction.Molecular analyses of the CCN2 promoter revealed that a highly conserved NF-κB binding site located within the CCN2 proximal promoter region is responsible for the activation of the promoter by stretch.Chromatin immunoprecipitation assays showed that NF-κB binds to the endogenous CCN2 promoter in both stretched cells and mechanically overloaded bladder tissues.Furthermore, inhibition of either actin polymerization or actin signaling-dependent pathways significantly altered the CCN2 promoter activity.Incubation of cultured SMCs with a cell-penetrating peptide containing the N-terminal sequence, Ac-EEED, of smooth muscle alpha-actin, altered both actin cytoskeleton organization and stretch-mediated nuclear relocation of NF-1B and subsequently, it reduced CCN2 promoter activity.Thus, mechanical stretch-induced changes in actin dynamics mediate NF-κB activation and induce CCN2 gene expression.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.002 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.001 |
| Open science | 0.001 | 0.002 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.107 | 0.052 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".