siRNA directed against c-Myc inhibits proliferation and downregulates human telomerase reverse transcriptase in human colon cancer Colo 320 cells
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Post-publication record
- Nature
- Retraction
- Reason
- Duplication of/in Article;
- Date
- 7/16/2009 0:00
- Flagged by OpenAlex?
- Yes
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Abstract
The c-Myc and human telomerase reverse transcriptase gene (hTERT) gene are frequently deregulated and overexpressed in malignancy. hTERT activity is induced by c-Myc and strategies designed to inhibit c-Myc expression in cancer cells may have considerable therapeutic value. We designed and used a short hairpin RNA to inhibit c-Myc expression in Colo 320 cells and validated its effect on cell proliferation. In this study, four c-Myc-shRNA expression vectors were constructed and introduced into Colo 320 cells. The effects of c-Myc silencing on tumor cell growth was assessed by soft agar assay and DNA synthesis experiments. The expressions of c-Myc and hTERT were also assessed by real-time reverse transcription-polymerase chain reaction and Western blot analysis. Upon transient transfection with plasmid encoding shRNA, it was found that expression of c-Myc and hTERT decreased in shRNA-transfected cells. The downregulation of c-Myc and hTERT inhibited cell growth, shortened telomere lengths, and suppressed telomerase activity. In conclusion, our findings demonstrate that shRNA of c-Myc can inhibit the DNA replication in Colo 320 cells effectively and reduce telomere length and telomerase activity, therefore, it could be used as a new potential anticancer tool for therapy of human colon cancer.
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The record
- Venue
- Journal of Experimental & Clinical Cancer Research
- Topic
- RNA Interference and Gene Delivery
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- University of British Columbia
- Funders
- —
- Keywords
- Telomerase reverse transcriptaseSmall hairpin RNATelomeraseTransfectionMolecular biologyTelomereCell growthGene silencingCancer researchBiologyRNA interferenceCancer cellChemistryCell cultureCancerRNAGeneGene knockdownGenetics
- Has abstract in OpenAlex
- yes