Difficulties Storing and Preserving Tyrant Flycatcher Blood Samples used for Genetic Analyses
Bibliographic record
Abstract
Abstract We stored blood samples of Eastern Phoebes (Sayornis phoebe) in a lysis buffer (“QLB”) that has been used successfully to preserve blood samples of many other species. We found that although samples from adults were not affected greatly, samples of nestling blood stored for more than a few days did not reliably produce the quantity and quality of DNA useful for multi-locus DNA fingerprinting. We also were unable to extract usable DNA from blood samples collected from Eastern Kingbird (Tyrannus tyrannus) nestlings, but obtained usable DNA from blood of Least Flycatcher (Empidonax minimus) nestlings stored for more than a year. We recommend that anyone planning DNA research with tyrant flycatchers should conduct their DNA extractions as soon as possible after collection. A pilot study to test methods of storage, preservation, and extraction may be necessary before beginning a large-scale project.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".