Experimental determination of net protein charge, [A]<sub>tot</sub>, and<i>K</i><sub>a</sub>of nonvolatile buffers in bird plasma
Bibliographic record
Abstract
The quantitative mechanistic acid-base approach to clinical assessment of acid-base status requires species-specific values for [A] tot (the total concentration of nonvolatile buffers in plasma) and K a (the effective dissociation constant for weak acids in plasma). The aim of this study was to determine [A] tot and K a values for plasma in domestic pigeons. Plasma from 12 healthy commercial domestic pigeons was tonometered with 20% CO 2 at 37°C. Plasma pH, Pco 2 , and plasma concentrations of strong cations (Na, K, Ca), strong anions (Cl, l-lactate), and nonvolatile buffer ions (total protein, albumin, phosphate) were measured over a pH range of 6.8–7.7. Strong ion difference (SID) (SID 5 = Na + K + Ca − Cl − lactate) was used to calculate [A] tot and K a from the measured pH and Pco 2 and SID 5 . Mean (±SD) values for bird plasma were as follows: [A] tot = 7.76 ± 2.15 mmol/l (equivalent to 0.32 mmol/g of total protein, 0.51 mmol/g of albumin, 0.23 mmol/g of total solids); K a = 2.15 ± 1.15 × 10 −7 ; and p K a = 6.67. The net protein charge at normal pH (7.43) was estimated to be 6 meq/l; this value indicates that pigeon plasma has a much lower anion gap value than mammals after adjusting for high mean l-lactate concentrations induced by restraint during blood sampling. This finding indicates that plasma proteins in pigeons have a much lower net anion charge than mammalian plasma protein. An incidental finding was that total protein concentration measured by a multianalyzer system was consistently lower than the value for total solids measured by refractometer.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".