Rho‐Kinase‐Mediated Suppression of KDR Current in Cerebral Arteries Requires an Intact Actin Cytoskeleton
Bibliographic record
Abstract
This study examined the role of the actin cytoskeleton in Rho‐kinase‐mediated suppression of the delayed rectifier K + (K DR ) current in cerebral arteries. Myocytes from rat cerebral arteries were enzymatically isolated and whole cell K DR currents monitored using conventional patch clamp electrophysiology. At +40 mV, the K DR current averaged 19.8 ± 1.6 pA/pF (mean ± SE) and was potently inhibited by uridine triphosphate (UTP; 30 µM). Consistent with past observations, K DR current suppression was blocked by the Rho‐kinase inhibitors H‐1152 (300 nM) and Y‐27632 (30 µM). Likewise, disruption of the actin cytoskeleton using cytochalasin D (10 µM) or latrunculin A (10 nM) abolished the ability of UTP to suppress K DR . In intact cerebral arteries, UTP induced actin polymerization in a Rho‐kinase‐dependent manner, suggesting that Rho‐mediated actin reorganization could play a role in the modulation of K DR current. Using pressure myography techniques, UTP‐induced depolarization and constriction of cerebral arteries were found to be significantly reduced following Rho‐kinase inhibition with H‐1152, and following the disruption of the actin cytoskeleton using cytochalasin D or latrunculin A. We conclude from our electrophysiological, biochemical, and functional observations that Rho‐kinase mediated alteration of the actin cytoskeleton likely underlies the inhibition of K DR current by UTP, ultimately contributing to the depolarization and constriction of cerebral arteries.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".