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Record W4240964351 · doi:10.22215/etd/2015-11159

NFE2L Regulation BY Endoplasmic Reticulum Stress

2015· dissertation· en· W4240964351 on OpenAlexaff
Magdalena Bugno

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics, phytochemicals, and oxidative stress
Canadian institutionsCarleton UniversityUniversity of Toronto
Fundersnot available
KeywordsNRF1Endoplasmic reticulumUnfolded protein responseOxidative stressCell biologyXBP1Reactive oxygen speciesTunicamycinMG132DithiothreitolChemistryGlutathioneProteasomeBiochemistryBiologyProteasome inhibitorMitochondrionMitochondrial biogenesisGene

Abstract

fetched live from OpenAlex

Cell survival requires adaptive responses to transient increases of reactive oxygen species (ROS) during stress.The nuclear factor-erythroid 2-like factors (Nrf1 and Nrf2) and Maf cofactors transactivate genes with antioxidant response elements (ARE) to coordinate distinct metabolic pathways following ROS.Nrf2 predominantly responds to oxidative stress and electrophiles to regulate glutathione biosynthesis, while Nrf1 regulates proteasome induction.This thesis shows nuclear accumulation of the longer forms of Nrf1 (p120-and p95-Nrf1) in response to ER stress mediated by tunicamycin (TUN), thapsigargin (THP), and dithiothreitol (DTT) in HEK293T cells.Nrf2, as opposed to Nrf1, was induced by the oxidative stressor antimycin A (AA) in the absence of BiP induction while both Nrfs accumulated in the nuclei from DTT-mediated redox stress.Cell stress was monitored using the H2DCFDA, MTT and PI assays.These results are the first to indicate Nrf1 responds to ER stress distinctly from Nrf2 that responds to mitochondrial ROS.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.013

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0040.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.251
Teacher spread0.245 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2015
Admission routes1
Has abstractyes

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Same topicGenomics, phytochemicals, and oxidative stressFrench-language works237,207