EphB6-null mutation results in compromised T cell function
Bibliographic record
Abstract
So far, there is very limited knowledge about the role of Eph kinases, the largest family of receptor tyrosine kinases, in the immune system.Here, using EphB6 -/-mice, we demonstrated that in vitro and in vivo T cell responses such as lymphokine secretion, proliferation, and the development of delayed-type skin hypersensitivity and experimental autoimmune encephalitis in EphB6 -/-mice were compromised.On the other hand, humoral immune responses, such as serum levels of different Ig isotypes and IgG response to tetanus toxoid, were normal in these mice.Mechanistically, we showed that EphB6 migrated to the aggregated TCRs and rafts after TCR activation.Further downstream, in the absence of EphB6, ZAP-70 activation, LAT phosphorylation, the association of PLCγ1 with SLP-76, and p44/42 MAPK activation were diminished.Thus, we have shown that EphB6 is pivotal in T cell function.1A.A segment starting from exon III, which contains the start codon until the end of intron IV in the EphB6 gene, was replaced by a GFP and neomycin expression cassette.Strain 129/sv embryonic stem cells were transfected with the targeting construct, selected with G418, and screened by PCR.Correctly targeted clones were injected into C57BL/6 blastocysts.Two chimeric mice were obtained; both achieved germ-line transmission.Offspring from 1 chimeric founder was expanded.Tail DNA was digested with HindIII and analyzed by Southern blotting Nonstandard abbreviations used: DP, double positive; DTH, delayed-type hypersensitivity; EAE, experimental autoimmune encephalitis; EFN, ephrin; Results Generation of EphB6 -/-mice. The targeting construct to generate EphB6 -/-mice is illustrated in Figure
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".