The splicing inhibitor Isoginkgetin leads to defects in multiple phases of the cell cycle
Bibliographic record
Abstract
Isoginkgetin is a naturally occurring compound from Ginkgo biloba trees that has anti-neoplastic activities related to inhibition of spliceosome assembly.The p53-tumour suppressor plays a prominent role in cell G1 and G2 cycle checkpoints and p53 is strongly activated in response to transcriptional stresses.Therefore, we determined the effect of p53 and isoginkgetin treatment on cell cycle progression.Wildtype p53 expressing cells exhibited a remarkable S phase defect while the p53-null subline accumulated with 4C DNA content.Despite the pronounced difference in these isogenic strains, there was no pronounced p53 transcriptional response as assessed by qRT-PCR and preliminary microarray analysis.Instead, isoginkgetin leads to altered pre-mRNA splicing of S and M phase-specific transcripts.These results suggest that cell cycle alterations are the direct result of abnormal splicing of cell cycle regulatory mRNAs and not p53 dependent checkpoints.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".