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Record W4251741141 · doi:10.31274/etd-20210609-33

Genetic basis of disease resilience in wean-to-finish pigs under a natural disease challenge model

2021· dissertation· en· W4251741141 on OpenAlexfundno aff
Jian Cheng

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenetic and phenotypic traits in livestock
Canadian institutionsnot available
FundersNational Institute of Food and AgricultureGenome AlbertaGenome CanadaGenome PrairieU.S. Department of Agriculture
KeywordsDiseaseNatural (archaeology)Resilience (materials science)Basis (linear algebra)BiologyMedicineMathematicsInternal medicinePhysics

Abstract

fetched live from OpenAlex

The overall objective of this thesis was to characterize the genetic basis of disease resilience traits in wean-to-finish pigs from a natural disease challenge model, including growth and carcass performance, clinical disease traits, and feed and water intake and behavior traits under a severe natural polymicrobial disease challenge. This thesis includes five manuscripts that have or will be submitted to be published in scientific journals. The first study aimed to estimate genetic parameters of growth and carcass performance and clinical disease traits under this disease challenge, to develop methods of including data on pigs that died before slaughter into genetic analysis of disease resilience, and to evaluate and validate the usefulness of traits derived from feed intake data and subjective health scores as genetic indicator traits for disease resilience. The second study continued to estimate genetic parameters for feed and water intake and behavior traits, to estimate the genetic correlations of these traits with growth rate and clinical disease traits, to evaluate the usefulness of day-to-day variation and proportion of off days derived from drinking behavior traits as indicators of disease resilience, and to develop other water and feed intake behavior indicator traits to select for disease resilience. These first two studies found that disease resilience traits, including treatment rates, mortality rates, and subjective health scores were lowly heritable, while growth and carcass performance, and feed and water intake and behavior traits under the disease challenge were moderate to highly heritable. Results also showed that resilience indicator traits derived from the feed and water intake patterns can be used for selection of more resilient pigs. Especially promising traits were day-to-day variation in feed intake duration and average daily number of water intake visits. The third study explored the opportunities of applying reaction norm models to identify disease resilient pigs. The first objective was to quantify the disease challenge load that pigs within a pen were exposed to, using growth rate and clinical disease phenotypes. The second objective was to quantify genetic variation in disease resilience using reaction norm models based on the developed challenge loads. The best challenge load for growth rate in the challenge nursery was the challenge load derived based on early growth rate in the finisher, while the challenge load derived from the clinical disease traits across the challenge nursery and finisher was the best challenge load for growth rate in the finisher and treatment rate in the challenge nursery and across challenge nursery and finisher. Reaction norm models were found to be able to identify the genetic variation across different disease challenge loads. The results of this study can be implemented to select more resilient animals across a range of challenge loads, or high-performing animals at a given challenge load, or a combination of these. The fourth study aimed to identify genomic regions that are associated with disease resilience using genome-wide association studies (GWAS) and fine mapping tools. The major histocompatibility complex (MHC) and several other quantitative trait loci (QTL) were identified. Four QTL were identified in the MHC region for growth rate in the challenge nursery by fine mapping, including one single nucleotide polymorphism (SNP) with major effects. These QTL play an important role in host response to infectious diseases and can be incorporated in selection to improve disease resilience. The final study developed an efficient leave-one-out cross-validation method for prediction of breeding values under a general mixed linear model with multiple random effects, which can be used to predict the accuracy of genomic prediction of disease resilience traits. In conclusions, disease resilience traits obtained under the natural disease challenge model were heritable and can, therefore, be selected for. Multiple important QTL, including MHC, were identified for disease resilience traits. Genetic indicators derived from feed and water intake patterns, reaction norm models using the developed challenge load, and QTL and genetic markers identified, along with genomic prediction, can be used to facilitate the genetic progress of disease resilience.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.259
Teacher spread0.248 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2021
Admission routes1
Has abstractyes

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