Investigating splicing in two disparate organisms: a highly reduced spliceosome in Cyandioschyzon Merolae and additional functionality in Saccharomyces Cerevisiae's PRP24
Bibliographic record
Abstract
Splicing, the removal of non-protein coding introns from pre-mRNA, is a critical step in eukaryotic gene expression that is facilitated by a large, dynamic ribonucleoprotein complex known as the spliceosome.The spliceosome is composed of five subcomponents of small nuclear ribonucleoprotein particles consisting of a snRNA and their associated proteins; these "snRNPs" are referred to as U1, U2, U4, U5, and U6.Recently, the red alga Cyanidioschyzon merolae was found to be lacking the U1 snRNA, raising questions of whether U1 proteins are also absent and to what extent the rest of the spliceosome is reduced.To address this, I computationally searched for known splicing proteins in C. merolae.In doing so, I found no U1 proteins and a highly reduced spliceosome consisting of 69 splicing proteins in the organism.Additionally, I performed several investigations into Prp24 functionality and I submit that, in addition to U6 binding through electropositive residues in Prp24's second RNA recognition motif, there exists a secondary function based on the presence of cold-sensitive phenotypes in the region.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".