A fast and efficient method to introduce apple latent spherical virus to legume plants via <scp><i>Agrobacterium rhizogenes</i></scp>‐mediated transformation of hairy roots
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Abstract Virus‐induced gene silencing (VIGS) is a functional genomics tool used to determine the function of unknown genes or assess the impact of gene silencing on plant phenotype. However, VIGS methods for analyzing gene function are not equally efficient across species, and virus inoculation is difficult for some legume species. We describe a fast and efficient inoculation method using Agrobacterium rhizogenes K599 harboring an apple latent spherical virus (ALSV) full‐length cDNA clone. Pisum sativum cv. AAC Lacombe and Lens culinaris cv. CDC Viceroy showed silencing rates of 100% and 47.7%, respectively, within 30 days starting from seed. To our knowledge, this is the first report of virus inoculation by A. rhizogenes ‐mediated introduction to legume plants. This work paves the way for high throughput gene function screening in Pisum sativum and other closely related legume crops using ALSV as a symptomless VIGS vector.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it