MétaCan
Menu
← Back to cohort
Record W4281938708 · doi:10.21203/rs.3.rs-1656990/v1

Apoptotic-like PCD inducing HRC gene when silenced enhances multiple disease resistance in plants

2022· preprint· en· W4281938708 on OpenAlexaff
Ajjamada C. Kushalappa, Hegde NG, Raghavendra Gunnaiah, Atul Sathe, Kalenahalli Yogendra, Lissa Ajjamada

Bibliographic record

VenueResearch Square · 2022
Typepreprint
Languageen
FieldAgricultural and Biological Sciences
TopicPlant-Microbe Interactions and Immunity
Canadian institutionsMcGill University
Fundersnot available
KeywordsBiologyEffectorPathogenHypersensitive responseGenePlant disease resistanceCell biologyCytosolInnate immune systemProgrammed cell deathApoptosisImmunityGeneticsImmune systemBiochemistryEnzyme

Abstract

fetched live from OpenAlex

Abstract Programed cell death (PCD) plays an important role in plant environmental stress and has the potential to be manipulated to enhance disease resistance. Plants have innate immunity and, following pathogen perception, the host induces a Hypersensitive Response PCD (HR-PCD), leading to pattern (PTI) or effector triggered immunity (ETI). Here we report a non-HR type or apoptotic-like PCD (AL-PCD) in pathogen infected wheat and potato. The pathogen induced Ca2+ in the apoplast is transported to the cytosol where it binds to calmodulin (CAM). The Ca2+-CAM binds to histidine-rich-calcium-binding-protein (HRC) increasing Ca2+ concentration in the cytosol. The HRC moves to the nucleus and increases Ca2+ concentration that triggers endonucleases which induce AL-PCD, but not when HRC is silenced. The absence of AL-PCD reduced food for pathogen which results in reduced biomass and mycotoxin accumulation. The reduced gene function inhibition, promotes expression of the R-gene repertoire of the host, leading to enhanced multiple pathogen resistance. The functional HRC in commercial varieties of different crops can be silenced by CRISPR-Cas9 genome editing to enhance resistance to multiple pathogens.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.077
GPT teacher head0.334
Teacher spread0.258 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2022
Admission routes1
Has abstractyes

Explore more

Same venueResearch Square→Same topicPlant-Microbe Interactions and Immunity→French-language works237,207→