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EXPLOITATION OF VARYING LIGHT REGIMES AND HIGH SUCROSE CONCENTRATIONS FOR IN VITRO TUBERIZATION IN POTATO: A STEP TOWARDS DISEASE FREE SEED PRODUCTION

2022· article· en· W4283763426 on OpenAlexfundno aff
Saher Ashraf, Ghulam Mustafa, Muhammad Sajjad Khan, Muhammad Aslam

Bibliographic record

VenuePakistan Journal of Phytopathology · 2022
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPotato Plant Research
Canadian institutionsnot available
FundersHigher Education Commision, PakistanHigher Education Commission, PakistanAlberta Agricultural Research Institute
KeywordsBiologySucroseProduction (economics)BotanyIndustrial cropIn vitroAgronomyHorticultureSolanaceaeFood scienceGeneticsGene

Abstract

fetched live from OpenAlex

Potato is the 4 th major vegetable crop in our country but more than 90% of the seed is imported which costs heavy economic burden on the foreign exchange. Further, imported germplasm is not well adapted to local climatic conditions and is vulnerable to different types of diseases. In vitro manipulations have proved their worth not only for the mass multiplication but also for the production of disease free seed of various crop plants. A proficient in vitro micropropagation and tuberization system was established in elite potato cultivars Kuroda and Desiree. In addition to growth hormones, sucrose concentration and light regime appeared to have dominant effect on microtuberization. Maximum number of microtubers was observed in genotype Desiree (2.20±0.20) followed by Kuroda (2.0±0.316) on MS medium augmented with 8% sucrose. Average fresh weight was maximum (0.70±0.063 g) on MS medium augmented with 10% sucrose in genotype Desiree. Likewise, best microtuberization was observed in initial 20 days light (16 hrs light with 8 hrs dark) incubation followed by complete dark incubation. The resultant microtubers were multiplied in pots for the production of minitubers and were tested for disease infestation by ELISA (Enzyme Linked Immunosorbant Assay). The antigen-antibody interaction was detected to be similar to the negative control (0.35-0.528) indicating absence of the viral pathogens. Hence, the developed protocol can be employed for the mass multiplication of disease free seed of elite potato cultivars resulting in reduced dependence on imported seed.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.889
Threshold uncertainty score0.152

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.266
Teacher spread0.248 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2022
Admission routes1
Has abstractyes

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