Cloning of <i>GABI5-3A3</i> Gene of and Functional Analysis in Tobacco
Bibliographic record
Abstract
ABI5 plays an important role in many biological processes such as seed dormancy and germination, growth and development, anthocyanin synthesis and response to stress. A large number of studies have shown that ABI5 is involved in seed dormancy and germination, while few studies have shown that ABI5 is involved in anthocyanin synthesis. In order to explore the involvement of ABI5 transcription factors in the regulation of wheat anthocyanin synthesis, the full cDNA length of Gz ABI5-3A3 was amplified from a color wheat variety ‘Guizimai 1’. Gz ABI5-3A3 may be involved in biological processes regulating plant growth, photosynthesis, flowering, seed germination and anthocyanin accumulation. Phylogenetic tree analysis showed that Gz ABI5-3A3 was homologous to Ta ABI5D-SH-31 , Ta ABI5D-SH-23 and Ta ABI5D-SW-23 in wheat. In this study, the overexpression vector of Gz ABI5-3A3 gene PBI121-Gz ABI5-3A3 was further constructed for tobacco genetic transformation. Eight tobacco transgenic lines were obtained by genetic transformation. Studies on Gz ABI5-3A3 overexpressed transgenic lines showed that the anthocyanin content in seedling leaves of transgenic tobacco lines L4, L7 and L15 was significantly lower than that of the wild type, and the expression levels of anthocyanin synthesis pathways of structural genes Nt PAL , Nt DFR , Nt ANS and Nt CHS were significantly decreased. The results showed that Gz ABI5-3A3 could negatively regulate anthocyanin synthesis by regulating the expression of structural genes in anthocyanin synthesis pathway. This study provides a research basis for the subsequent research on the molecular mechanism of Gz ABI5-3A3 regulating anthocyanin synthesis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".