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Record W4288036018 · doi:10.21203/rs.3.rs-1852161/v1

Estimation of distance distributions between Gd3+ radical pairs with a significant zero- field splitting from pulsed EPR DEER data

2022· preprint· en· W4288036018 on OpenAlexafffund
Sushil K. Misra, Hamid Reza Salahi

Bibliographic record

VenueResearch Square · 2022
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicElectron Spin Resonance Studies
Canadian institutionsConcordia University
FundersNatural Sciences and Engineering Research Council of CanadaWeizmann Institute of Science
KeywordsTikhonov regularizationElectron paramagnetic resonanceKernel (algebra)Density matrixDistribution (mathematics)PhysicsChemistryAtomic physicsMathematicsNuclear magnetic resonanceMathematical analysisQuantum mechanicsCombinatoricsInverse problem

Abstract

fetched live from OpenAlex

Abstract The numerical method, using full diagonalization of the spin-Hamiltonian matrix, to calculate DEER (Double Electron-Electron Resonance) signal, based on the double rotating-frames (DRF) technique, taking into account the zero-field splitting of the Gd 3+ ion with spin S=7/2 is exploited here to calculate the kernel signals for the various distances, r, between two coupled Gd 3+ radicals, distributed randomly in a biological system. These are then used to estimate the probabilities of the distance distribution, P ( r ), between the various Gd 3+ radical pairs, separated by the distances, r . This is accomplished by using Tikhonov regularization, as implemented in the software DeerAnalysis [Jeschke et al. Appl. Magn. Reson., 30(3), pp.473-498 (2006)], but using the kernel signals calculated here by the DRF technique for different r values. This procedure is successfully illustrated by applying it to calculate the distance distribution probabilities, P ( r ) from the reported experimental four-pulse DEER data for a sample of Gd ruler 1_5 in D 2 O/glycerol-d_8 (i) at Q-band [Doll et al., J. Magn. Reson. 259, pp.153-162] and (ii) at W-band [Dalaloyan et al., Phys. Chem. Chem. Phys., 17(28), pp.18464-18476 (2015)]. Significant differences in the distance-distribution probabilities are found between those calculated here using the DRF-calculated kernel signals for Gd 3+ with spin 7/2 with significant ZFS from those calculated by the use of analytical kernel signals for spin-½ system without ZFS, using hard pulses.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.323
Threshold uncertainty score0.937

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.003
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.062
GPT teacher head0.394
Teacher spread0.332 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2022
Admission routes2
Has abstractyes

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