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Regeneration of pitaya by indirect organogenesis evaluated by scanning electron microscopy and flow cytometry

2022· article· en· W4288063127 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenuePesquisa Agropecuária Brasileira · 2022
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicBotanical Research and Applications
Canadian institutionsUniversity of Guelph
FundersFundação de Amparo à Pesquisa do Estado de Minas GeraisConselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
KeywordsOrganogenesisCallusExplant cultureScanning electron microscopeFlow cytometryBiologyHorticultureBotanyChemistryMolecular biologyMaterials scienceBiochemistryIn vitro

Abstract

fetched live from OpenAlex

Abstract The objective of this work was to evaluate the induction of indirect organogenesis by concentrations of dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ) in pitaya (Hylocereus undatus) explants, using scanning electron microscopy and the flow cytometry technique. The treatments consisted of the concentrations of 0, 2.0, and 4.0 mg L-1 2,4-D and TDZ and of the combinations of these regulators. Percentages of callus coverage at 45 and 60 days were evaluated. The explants subjected to the treatments were analized by flow cytometry and scanning electron microscopy. All treatments induced endoreduplication, and there was no somaclonal variation. Under the combination of 2.0 mg L-1 TDZ and 4.0 mg L-1 2,4-D, calluses were formed in 95% of the explants, but were smaller than those produced with 2,4-D separately. The concentration of 2.0 mg L-1 TDZ induces the indirect organogenesis in pitaya explants, confirmed by the presence of conducting vessels through scanning electron microscopy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.065
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.276
Teacher spread0.257 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it