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Record W4288262079 · doi:10.5281/zenodo.3369934

Supplementary data for "Evaluation of methods to assign cell type labels to cell clusters from single-cell RNA-sequencing data" (Diaz-Mejia JJ et al, 2019)

2019· article· en· W4288262079 on OpenAlexaff
J. Javier Díaz-Mejía

Bibliographic record

VenueZenodo (CERN European Organization for Nuclear Research) · 2019
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSingle-cell and spatial transcriptomics
Canadian institutionsUniversity Health Network
Fundersnot available
KeywordsRNA-SeqCellComputational biologyRNAComputer scienceBiologyGeneticsGeneGene expressionTranscriptome

Abstract

fetched live from OpenAlex

Five scRNA-seq datasets from human liver cells (MacParland et al, 2018), mouse retinal neurons (Shekhar et al, 2016), Tabula Muris (Tabula Muris Consortium, 2018) and two peripheral blood mononuclear cell (PBMCs) datasets (Zheng et al, 2017) and (Gierahn et al., 2017) were processed and curated as described by Diaz-Mejia et al 2019 to use them as inputs for benchmarking of cell cluster labeling methods. Five types of files are provided: a) Single-cell RNA-sequencing (scRNA-seq) cell cluster average gene expression matrices (*E_xy_matrix.tsv) b) Cell type gene expression signatures in the form of gene sets (*gmt), binary (*binary_profile.tsv) and continuous profiles (*continuous_profile.tsv) c) Reference cell type gold standard annotations (*gold_standards.tsv) d) Supplementary Table 1, containing ROC AUC, PR AUC and computing times reported by Diaz-Mejia et al 2019 in Figure 6. e) Supplementary Table 2, containing ROC AUC, PR AUC analysis of the PBMC datasets using either the LM22 (Newman et al 2015) or the Monaco et al (2019) cell type signatures. Note: as described in Diaz-Mejia et al 2019, the PBMC and Tabula Muris datasets were analyzed in two ways each: i) pbmc-22-10x and pbmc-6-10x, ii) pbmc-22-seqwell and pbmc-6-seqwell, and iii) tabula_muris_11 and tabula_muris_6.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.377
Threshold uncertainty score0.998

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0020.002
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.135
GPT teacher head0.346
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2019
Admission routes1
Has abstractyes

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