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Length-Based Substructure Mutation Policies for Improved RNA Design in Simulated Annealing

2022· article· en· W4293519391 on OpenAlexafffund
Ryan McBride, Herbert H. Tsang

Bibliographic record

Venuenot available
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA and protein synthesis mechanisms
Canadian institutionsTrinity Western UniversityWestern University
FundersNatural Sciences and Engineering Research Council of CanadaTrinity Western University
KeywordsSubstructureRNASimulated annealingComputer scienceComputational biologyAlgorithmMutation testingNucleic acid structureMutationTheoretical computer scienceGeneticsBiologyEngineering

Abstract

fetched live from OpenAlex

RNA Design is a crucial bioinformatics problem to tailor specific RNA sequences into structures that guide our biology and medicine. Because these computer generated sequences are often statistically different from observed RNA sequence and do not fold as intended in real lab conditions, methods are developed to ensure more biological consistency. One approach is substructure restriction where any solutions are composed of observed RNA substructures recorded in a database. However, studies on this restricted problem are limited: while our Applied Research Lab's Simulated Annealing solution (SIMARD) uses substructures it only considers a single mutation policy and single method of generating substructure consistent sequences. We therefore propose two new policies of mutation: uniform, randomly modifying any structure, and length proportional, substructures are swapped randomly in proportion to their RNA length to target substructures that cover the most bases of a problem. In experiments on roughly fifty RNA Design problems, we conclude the potential value of these substructure based mutation methods resulting in solutions potentially hundreds of bases closer to the target folded structure than previous Simulated Annealing solutions.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.009
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Simulation or modeling · Consensus signal: Simulation or modeling
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.003
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.009
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.257
Teacher spread0.240 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designSimulation or modeling
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2022
Admission routes2
Has abstractyes

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