Angiogenesis Following Three-Dimensional Culture of Isolated Human Endometrial Stromal Cells
Bibliographic record
Abstract
Background Endometriosis is the presence of endometrial tissue outside of the uterine cavity and is the most common gynecologic disorder in women of reproductive age. We have preliminary evidence that in the presence of a 3-dimensional (3-D) fibrin matrix, human endometrial glands, stroma, and neovascularization can develop in vitro, mimicking the earliest stages of endometriosis. The aim of the present study was to determine if angiogenesis can be developed in a 3-D culture of human stromal cells in vitro. Materials and methods This was an in vitro study of human endometrial biopsies in 3-D culture of fibrin matrix and conducted at a university affiliated infertility center. Biopsies were taken from ten normal ovulating women undergoing infertility treatment. The samples obtained from fundus of the uterine cavity were minced, stromal cells isolated and placed in a 3-D fibrin matrix culture system. Degree of proliferation of stromal cells, invasion of the fibrin matrix, gland formation, vessel sprouting and immunohistochemical characterization of cellular components were recorded. Results Three-dimensional culture of human stromal cells formed sheets of cells in the fibrin matrix. By 3-4 weeks, endothelial cell branching was observed and rudimentary capillary-like structures formed and endothelial cells confirmed by CD31 immunostaining. Conclusion These data show that stromal cells from endometrial explants can proliferate and invade a fibrin matrix in vitro generating new vessels. This procedure represents a controlled, quantifiable model for the study of angiogenesis during the menstrual cycle, and in conditions such as endometriosis and cancer.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".