Development of novel clostridial fusants for enhanced biobutanol production
Bibliographic record
Abstract
Biobutanol, a fuel with higher energy content than ethanol was produced in the present work through Simultaneous Saccharification and Fermentation (SSF) of wheat straw (WS) that traditionally produces acetone, butanol and ethanol solvents (ABE). Novel Clostridium strains were developed and tested for enhanced biobutanol production. Thermal stability was imparted to two mesophilic clostridial wild strains through protoplast fusion with that of a corresponding thermophilic clostridial strain. This novel development eliminated the need to add external enzymes during the SSF process, allowed for SSF to be conducted at an elevated temperature of 45°C, enhanced the activity of internally produced enzymes and tolerated butanol toxicity to new limits. In order to determine the stability of the genetically modified strains, the parent fusants were passed on to five successive growth cycles. The fused strains of each growth cycle were inoculated with substrate and examined for solvent production and compared to the parent generation. Polymerase Chain Reaction Technique (PCR) followed by Agarose Gel Electrophoresis demonstrated reproducible genetic stability of the fused strains. Highest biobutanol production of 13.8 g/L (almost twice the concentration reported by other laboratory scale batch SSF research studies) was produced by one of the novel fusants. Generally, fused strain that achieved the highest biobutanol production exhibited relatively constant butanol concentration over the five growth cycles. Optimistic results obtained from batch SSF at lab scale demonstrate a clear potential of these novel strains to improve productivity and yield of biobutanol at a large-scale facility.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".