Evaluación de tres protocolos de tratamiento hormonal sobre el diámetro de ovocitos de sabaleta Brycon henni Avaliação de tres protocolos de tratamento hormonal sob o diámetro dos ovocitos da sabaleta Brycon henni Evaluation of three protocols hormone treatment on the size of the oocytes of sabaleta Brycon henni
Bibliographic record
Abstract
El estudio fue realizado en el Municipio de San Carlos (Antioquia, Colombia), por la Facultad de Ciencias Agrarias de la Universidad de Antioquia, en convenio con la Corporación CORNARE, ISAGEN, durante la temporada reproductiva Mayo-Julio de 2008. 12 hembras y 24 machos de sabaleta Brycon henni de aproximadamente 100 g de peso corporal y 20 cm de longitud estándar, provenientes de un lote de 320 reproductores capturados del ambiente natural en Agosto del 2007 y mantenidos en cautiverio en un estanque de tierra de 200 m², fueron seleccionados por sus características externas de madurez sexual como vientre abultado, papila genital enrojecida y expulsión de semen después de leve presión sobre la cavidad celómica. Además como carácter de dimorfismo sexual se consideró para las hembras la presencia de aleta anal lisa o con espinaciones en máximo el 50% de su longitud, mientras que en los machos es rugosa en toda su extensión. También se tuvieron en cuenta características internas, en las hembras se realizó biopsia ovárica por aspiración de una muestra de ovocitos para observar la posición de sus núcleos y medición del de diámetro.. Las hembras fueron seleccionadas con la condición que presentaran ovocitos con diámetros superiores a 900 µm. Los animales fueron distribuidos para recibir uno de tres tratamientos, T1 Extracto de Hipófisis de Carpa (Argent, USA) (EHC), n=4; T2 Ovopel (Sigma Co., St Louis, Missouri), n=4; T3 Ovaprim (Syndel Laboratories Ltd, Vancouver, BC Canadá), n=4, durante cuatro fechas de muestreo y fertilizadas cada una con el semen de dos machos, inyectados previamente con EHC. 11 de las 12 hembras respondieron a los tratamientos y presentaron diámetros ovocitarios promedio de 1428.8 y 1679.8 (T1), 1531.2 y 1879.2 (T2) y de 1712.8 a 1803.3 (T3), antes y después del tratamiento hormonal, respectivamente. Se observaron diferencias significativas al interior del T1 y T2 (p<0.05), pero no en el T3. La distribución de frecuencias del diámetro de los ovocitos presentó en T1, dos modas antes del tratamiento hormonal y dos después del tratamiento hormonal (bimodal); T2, dos modas antes y una después (bimodal y unimodal) y T3, dos antes y dos después (bimodal). Los porcentajes de fertilización fueron bajos en el T1, pero se presentaron altos porcentajes en T2 y T3 hasta las 12 h de incubación de los huevos. O estudo foi realizado no Município de San Carlos (Antioquia, Colombia), pela Facultad de Ciencias Agrarias de la Universidad de Antioquia, conjuntamente com a corporação CORNARE, ISAGEN, entre os meses de maio e julho de 2008. Foram utilizadas 12 fêmeas e 24 machos de sabaleta Brycon henni de aproximadamente 100 g de peso corporal e 20 cm de comprimento, provenientes de um lote de reprodutores capturados em ambiente natural em agosto de 2007. Os animais receberam um dos três tratamentos: T1 extrato de hipófise de Hipófise de Carpa (Argent, USA) (EHC), n=4; T2 Ovopel (Sigma Co., St Louis, Missouri), n=4; T3 Ovaprim (Syndel Laboratories Ltd, Vancouver, BC Canadá), n=4, durante quatro datas de amostragem e fertilizadas cada uma com sêmen de dois machos, injetados previamente com EHC. 11 das 12 fêmeas responderam aos tratamentos e apresentaram ovócitos entre 1428.8 e 1679.8 (T1), 1531.2 e 1879.2 (T2) e de 1712.8 a 1803.3 (T3) de diâmetro, antes e depois do tratamento hormonal, respectivamente. Observaram-se diferencias significativas ao interior do T1 e T2 (p<0.05), mas não para o T3. A distribuição de freqüências do diâmetro do ovócito apresentou no T1, duas modas antes do tratamento hormonal e dois depois do tratamento hormonal (bimodal); T2, duas modas antes e 1 depois (bimodal e unimodal) e T3, duas antes e duas depois (bimodal). As porcentagens de fertilização foram baixas em T1, e foram altas em T2 e T3 até as 12 h de incubação dos ovos. The evaluation of three hormone treatments on the diameter size of ovocytes in Sabaleta was conducted during the breeding season from May to July 2008. Twelve Brycon henni females and 24 males, 100 g and a standard length of 24 cm, were selected according to their sexual maturity and external features such as bulging belly, red genital papilla and semen expulsion after softly pressing its coelomic cavity. The fish were selected from a 320 brood fish batch caught in the wild in August 2007 and kept in captivity in a 200 m² earth pond. The presence of an anal fin with or without spines of a maximum length of 50% was considered as sexual dimorphism in females. Internal characteristics were also considered; e.g., an ovarian aspiration biopsy was performed in females in order to observe its nuclei position and diameter. Ovocytes from selected females were at least 900 µm in diameter. Animals were distributed into three treatment groups for four sampling dates: T1 carp pituitary extract (CPE) (Argent Chemical Laboratories Inc., USA), n = 4; T2 Ovopel (Sigma Co. USA), n = 4; and T3 Ovaprim (Syndel Laboratories Ltd., Canada), n = 4. Each female was fertilized with the semen of two males previously injected with CPE. 11 out of 12 females had a treatment response, and resulting ovocyte average diameters were 1428.8 and 1679.8 (T1), 1531.2 and 1879.2 (T2), and 1712.8 to 1803.3 (T3), before and after hormone treatment, respectively. Significant differences were found within T1 and T2 (p <0.05); however, this was not the case for T3. For T1, frequency distribution of the diameter of ovocytes before the hormonal treatment was bimodal. T2 had two modes before and one after the treatment (bimodal and unimodal), and T3 had two before and two after (bimodal). Fertilization rates were low in T1; nevertheless, T2 and T3 had high rates until 12 h eggs incubation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".