Zasp52 strengthens whole embryo tissue integrity through supracellular actomyosin networks
Bibliographic record
Abstract
Abstract During morphogenesis, large scale changes of tissue primordia occur that are all coordinated across an embryo. In Drosophila , several tissue primordia and embryonic regions are bordered or encircled by supracellular actomyosin cables, junctional actomyosin enrichments networked between many neighbouring cells. We show that the single Drosophila Alp/Enigma family protein Zasp52, which is most prominently found in Z-discs of muscles, is a component of many supracellular actomyosin structures during embryogenesis, including the ventral midline and the boundary of the salivary gland placode. Zasp52, we uncover, contains within its central coiled-coil region a type of actin-binding motif usually found in CapZbeta proteins, and this domain displays actin binding activity. Using endogenously-tagged lines we identify that Zasp52 interacts with a subset of junctional components, including APC2, Polychaetoid/ZO-1, and Sidekick, as well as actomyosin regulators. Analysis of zasp52 mutant embryos revealed that the severity of the embryonic defects observed scales inversely with the amount of functional protein left. Large tissue deformations occur at sites where actomyosin cables are found during embryogenesis, suggesting a model whereby supracellular cables containing Zasp52 aid to insulate morphogenetic changes from one another. This is further supported by the analysis of in vivo processes as well as by an in silico vertex model of morphogenetic domains separated by a stiffness boundary.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".