Correction: The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamics
Bibliographic record
Abstract
The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamicsJournal of Biological ChemistryVol. 298Issue 9PreviewAutosomal recessive spastic ataxia of Charlevoix-Saguenay is a fatal brain disorder featuring cerebellar neurodegeneration leading to spasticity and ataxia. This disease is caused by mutations in the SACS gene that encodes sacsin, a massive 4579-amino acid protein with multiple modular domains. However, molecular details of the function of sacsin are not clear. Here, using live cell imaging and biochemistry, we demonstrate that sacsin binds to microtubules and regulates microtubule dynamics. Loss of sacsin function in various cell types, including knockdown and KO primary neurons and patient fibroblasts, leads to alterations in lysosomal transport, positioning, function, and reformation following autophagy. Full-Text PDF Open Access The author list should read as follows: Vincent Francis1, Walaa Alshafie1, Rahul Kumar1, Martine Girard1, Eric Krochmalnek1, Bernard Brais1, Peter S. McPherson2 The author contributions should read as follows: Author contributions - V. F., B. B., and P. S. M. conceptualization; V. F. and P. S. M. methodology; V. F. and P. S. M. writing–original draft; V. F., W. A., R. K., and M. G. visualization; V. F., W. A., R. K., M. G. and E.K. investigation; V. F., W. A., R. K., and M. G. validation; B. B. and P. S. M. supervision; V. F. and P. S. M. writing–review and editing; R. K. software; V. F. and P. S. M. data curation.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.025 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.002 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.002 | 0.002 |
| Scholarly communication | 0.003 | 0.002 |
| Open science | 0.003 | 0.001 |
| Research integrity | 0.007 | 0.008 |
| Insufficient payload (model declined to judge) | 0.062 | 0.024 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".