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Record W4311680946 · doi:10.22215/etd/2022-15248

Development of peptides for use as antimicrobial agents

2022· dissertation· en· W4311680946 on OpenAlexaff
Ali Shukri

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldImmunology and Microbiology
TopicAntimicrobial Peptides and Activities
Canadian institutionsCarleton University
Fundersnot available
KeywordsAntimicrobialAntimicrobial peptidesPeptideComputational biologyBiologyAntibiotic resistanceMicrobiologyBiochemistryAntibiotics

Abstract

fetched live from OpenAlex

Pathogenic bacteria are evolving resistance to conventional therapeutics at a rate which threatens our ability to reliably treat common infections, necessitating the discovery of new therapeutics.In this thesis, I look at the development of novel peptides that can be used to combat antimicrobial resistance.In Chapter 2, I employ a permutation of two known antimicrobial peptides (AMPs), Indolicidin and UyCT3, and through sequential generations of evolution, develop a peptide that can inhibit bacterial growth better than the wild type AMP.These AMPs are tested on clinically derived strains to help translate the clinical relevance of these findings.In Chapter 3, I use an orientated peptide array library (OPAL) to assist in the discovery of peptide -lactamase inhibitors against the -lactamase TEM-1.Candidates' activities were assessed for inhibition against TEM-1.These results show the significance of our findings and the robustness of the techniques that can be used for the discovery of peptide antimicrobials.I would first like to express my most sincere gratitude to both of my supervisors, Dr. Kyle Biggar, and Dr. Alex Wong.I knew being co-supervised would be a challenge but having both of you as my supervisors was an honour.To Alex: thank you for accepting me into your lab and taking

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0030.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.028
GPT teacher head0.283
Teacher spread0.255 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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