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Record W4311680960 · doi:10.22215/etd/2022-15148

Wastewater Surveillance of SARS-CoV-2 at a Canadian University Campus and the Impact of Wastewater Characteristics on Viral RNA Detection.

2022· dissertation· en· W4311680960 on OpenAlexafffundabout
Lena Bitter

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldMedicine
TopicSARS-CoV-2 detection and testing
Canadian institutionsCarleton University
FundersMinistère de l’Environnement, de la Protection de la nature et des ParcsMinistry of Environment
KeywordsWastewaterUltrafiltration (renal)Environmental scienceSanitary sewerResidenceSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Sewage treatmentSewageEnvironmental engineeringWaste managementEngineeringChromatographyChemistryMedicine

Abstract

fetched live from OpenAlex

Because of the increased population\ndensity, high-risk behavior\nof young students, and lower vaccination rates, university campuses\nare considered hot spots for COVID-19 transmission. This study monitored\nthe SARS-CoV-2 RNA levels in the wastewater of a Canadian university\ncampus for a year to provide actionable information to safely manage\nCOVID-19 on campus. Wastewater samples were collected from the campus\nsewer and residence buildings to identify changes, peaks, and hotspots\nand search for associations with campus events, social gatherings,\nlong weekends, and holidays. Furthermore, the impact of wastewater\nparameters (total solids, volatile solids, temperature, pH, turbidity,\nand UV absorbance) on SARS-CoV-2 detection was investigated, and the\nefficiency of ultrafiltration and centrifugation concentration methods\nwere compared. RT-qPCR was used for detecting SARS-CoV-2 RNA. Wastewater\nsignals largely correlated positively with the clinically confirmed\nCOVID-19 cases on campus. Long weekends and holidays were often followed\nby increased viral signals, and the implementation of lockdowns quickly\ndecreased the case numbers. In spite of online teaching and restricted\naccess to campus, the university represented a microcosm of the city\nand mirrored the same trends. Results indicated that the centrifugation\nconcentration method was more sensitive for wastewater with high solids\ncontent and that the ultrafiltration concentration method was more\nsensitive for wastewater with low solids content. Wastewater characteristics\ncollected from the buildings and the campus sewer were different.\nStatistical analysis was performed to manifest the observations. Overall,\nwastewater surveillance provided actionable information and was also\nable to bring high-risk factors and events to the attention of decision-makers,\nenabling timely corrective measures.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.045
Threshold uncertainty score0.878

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.267
Teacher spread0.251 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2022
Admission routes3
Has abstractyes

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